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Article10.1096/fasebj.29.1_supplement.lb727

Electrophysiological Properties of Magno‐and Parvo‐Cellular Neurons in Rat Hypothalamic Paraventricular Nucleus

Yi Ming Shen,So Yeong Lee,Seong Kyu Han,Pan Dong Ryu-2015-04-01-The FASEB Journal
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TL;DRAbstract

The hypothalamic paraventricular nucleus (PVN) contains magnocellular neurosecretory neurons (PVN‐MC) projecting to posterior pituitary, and parvocellular neurons projecting to anterior pituitary (neurosecretory), medulla (PVN‐RVLM) and/or spinal cord (PVN‐IML) (presympathetic). These PVN neurons are different in their roles and projections, but the differences in excitability are not well known. In this study, we compared the basic electrophysiological properties of these neuron groups. The PVN‐MC, PVN‐IML and/or PVN‐RVLM neurons were identified by electrophysiological property (expressing a prominent transient outward rectification) and retrograde dye (FluoSphere‐Red). The spontaneous firing, EPSC and action potential were recorded in the cell‐attached or whole‐cell mode with Axopatch 200B. Data were sampled at 20 KHz using pClamp 8 data‐acquisition and analysis software. In the voltage clamp cell‐attached mode, the frequency of spontaneous firing in the PVN‐IML neurons was higher th

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The hypothalamic paraventricular nucleus (PVN) contains magnocellular neurosecretory neurons (PVN‐MC) projecting to posterior pituitary, and parvocellular neurons projecting to anterior pituitary (neurosecretory), medulla (PVN‐RVLM) and/or spinal cord (PVN‐IML) (presympathetic). These PVN neurons are different in their roles and projections, but the differences in excitability are not well known. In this study, we compared the basic electrophysiological properties of these neuron groups. The PVN‐MC, PVN‐IML and/or PVN‐RVLM neurons were identified by electrophysiological property (expressing a prominent transient outward rectification) and retrograde dye (FluoSphere‐Red). The spontaneous firing, EPSC and action potential were recorded in the cell‐attached or whole‐cell mode with Axopatch 200B. Data were sampled at 20 KHz using pClamp 8 data‐acquisition and analysis software. In the voltage clamp cell‐attached mode, the frequency of spontaneous firing in the PVN‐IML neurons was higher th

Keywords

Parvocellular cellElectrophysiologyRostral ventrolateral medullaInternal medicineNucleusChemistryNeuroscienceSpinal cord

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