Identification and validation of SSR markers for Karnal bunt (Neovossia indica) resistance in wheat (Triticum aestivum)

TL;DRAbstract

Present study was carried out to screen 107 recombinant inbred lines (RILs) of cross H567.71 (resistant) × WH 542 (susceptible) for Karnal bunt [Neovossia indica (Mitra) Mundkar] resistance and to identify and validate SSR markers associated with Karnal bunt resistance in wheat (Triticum aestivum L.). There was significant variation among the recombinant inbred lines for percentage and coefficient of infection to Karnal bunt. Most of the RILs (85) and (72) showed up to 5% infection during the year 2010 and 2011 and thus were resistant. The range of percentage of infection (PI) in the susceptible RILs was 5.19 to 22.93 and 5.38 to 36.46 in the year 2010 and 2011 respectively, whereas coefficient of infection on RILs ranged from 0 to 8.28 and 0 to 14.45 in the year 2010 and 2011 respectively. A total of 70 SSR markers were used to screen parental genotypes out of these 42 were polymorphic. Total of 88 alleles were detected, number of alleles per locus ranged from 2-3 with an average of 2

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