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Intrinsic Bioprobes, Inc. (Tempe, AZ)

Randall W. Nelson,Peter Williams,Jennifer R. Krone-2008-07-15-OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information)

TL;DRAbstract

Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.

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Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.

Keywords

AnalyteChromatographyChemistryImmunoassayMass spectrometrySpecific antibodyAnalytical Chemistry (journal)Antigen

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