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Выделение гликопротеида из фиксированного вируса бешенства штамма «Москва 3253» и конструирование на его основе диагностикума для дот-иммуноанализа

Шарапова Наталия Анатольевна,Киреев Михаил Николаевич,Абрамова Елена Геннадьевна,Генералов Сергей Вячеславович,Матвеева Жанна Владимировна,Жулидов Иван Михайлович+5 more-2012-01-01-CyberLeninK (CyberLeninka)
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TL;DRAbstract

Described here are the results of glicoproteid isolation from the fixed rabies virus, strain «Moscow 3253», using non-ionic detergent with subsequent chromatographic purification. The obtained antigen was demonstrated to be applicable as immunoreagent for construction of diagnosticum, by means of conjugation with colloid gold nanoparticles. The diagnosticum is meant for detection of specific antibodies in immune sera of horsesproducers, and in the preparation of anti-rabies immunoglobulin, in dot-immunoassay.

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Described here are the results of glicoproteid isolation from the fixed rabies virus, strain «Moscow 3253», using non-ionic detergent with subsequent chromatographic purification. The obtained antigen was demonstrated to be applicable as immunoreagent for construction of diagnosticum, by means of conjugation with colloid gold nanoparticles. The diagnosticum is meant for detection of specific antibodies in immune sera of horsesproducers, and in the preparation of anti-rabies immunoglobulin, in dot-immunoassay.

Keywords

ImmunoassayRabies virusAntigenAntibodyChromatographyChemistryVirusVirology

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