Chromogenic and Accelerated Cultural Methods

TL;DRAbstract

Analysis of food samples by cultural and enrichment techniques remains an integral part of the examination of food for the presence or enumeration of foodborne pathogens. Enrichment methods have afforded the ability to detect as few as one cell per 500 g of food. In addition, enrichment techniques aid in the recovery of injured bacteria, which are often present in foods in a stressed condition because some foods may lack the optimal nutrients essential for bacterial growth, the food may present an environment not conducive to growth of the microorganisms, or the bacteria may be damaged during processing of the food. In this regard, the enrichment process provides an optimal growth medium and a period of time that can be effectively used by injured/stressed microorganisms to perform repair of cellular damage, allowing for resuscitation of the bacterial cells. Additionally, enrichment processes provide an opportunity for target bacterial cells to proliferate, while the growth of competin

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