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Article10.1117/12.2183161

Non-linear imaging techniques visualize the lipid profile of C.<i>elegans</i>

Meropi Mari,Barbara Petanidou,Konstantinos Palikaras,C. Fotakis,Nektarios Tavernarakis,George Filippidis-2015-07-14-Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE
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TL;DRAbstract

The non-linear techniques Second and Third Harmonic Generation (SHG, THG) have been employed simultaneously to record three dimensional (3D) imaging and localize the lipid content of the muscular areas (ectopic fat) of <i>Caenorhabditis elegans (C. elegans). </i>Simultaneously, Two-Photon Fluorescence (TPEF) was used initially to localize the stained lipids with Nile Red, but also to confirm the THG potential to image lipids successfully. In addition, GFP labelling of the somatic muscles, proves the initial suggestion of the existence of ectopic fat on the muscles and provides complementary information to the SHG imaging of the pharynx. The ectopic fat may be related to a complex of pathological conditions including type-2 diabetes, hypertension and cardiovascular diseases. The elucidation of the molecular path leading to the development of metabolic syndrome is a vital issue with high biological significance and necessitates accurate methods competent of monitoring lipid storage distr

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The non-linear techniques Second and Third Harmonic Generation (SHG, THG) have been employed simultaneously to record three dimensional (3D) imaging and localize the lipid content of the muscular areas (ectopic fat) of <i>Caenorhabditis elegans (C. elegans). </i>Simultaneously, Two-Photon Fluorescence (TPEF) was used initially to localize the stained lipids with Nile Red, but also to confirm the THG potential to image lipids successfully. In addition, GFP labelling of the somatic muscles, proves the initial suggestion of the existence of ectopic fat on the muscles and provides complementary information to the SHG imaging of the pharynx. The ectopic fat may be related to a complex of pathological conditions including type-2 diabetes, hypertension and cardiovascular diseases. The elucidation of the molecular path leading to the development of metabolic syndrome is a vital issue with high biological significance and necessitates accurate methods competent of monitoring lipid storage distr

Keywords

Caenorhabditis elegansNile redMolecular imagingMicroscopyLipid dropletAutofluorescenceFluorescenceBiophysics

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