Ion-exchange capillary electrochromatography of inorganic and small organic anions

TL;DRAbstract

This work presents a systematic study on the use of packed column ion-exchange capillary electrochromatography (IE-CEC) for the separation of ionic analytes. Methods have been developed for preparing packed capillary columns utilising either silica-based or polymer-based stationary phases. The stationary phase is retained by futs prepared by sintering either the silica-based packing material or pure silica. The electroosmotic and pressure-driven properties of frits prepared from bare or functionalised silica were investigated. Despite the high temperature of heating of the packing, sufficient residual functional groups remained on the fut such that distinctively different EOF behaviour was observed for each type of packing. Frits made from materials providing negative surface charges increased the magnitude of the cathodic EOF compared to the open capillary, but a substantial anodic EOF of -1.88 10-8 m2 v-1 s-1 was produced by introduction of a single fut made from SAX material. An exp

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