Translational control by the ribosomal protein Asc1p/Cpc2p in Saccharomyces cerevisiae

TL;DRAbstract

The mRNA-specific control of translation is of great importance for the adjustment to environmental changes, as e.g. amino acid limitations. It is especially mediated through the mRNA-5 untranslated regions (5 UTRs), containing a variety of translationally regulatory elements. This work presents a method to identify and analyze translationally regulative 5 UTRs (5 TRU) in Saccharomyces cerevisiae. Herein, a de novo proteome-based approach in conjunction with transcriptome data revealed thirteen proteins, whose biosynthesis is up-regulated post-transcriptionally under amino acid starvation conditions. The assessment of the corresponding 5 UTRs by a newly developed testing system proposes a translational up-regulation upon amino acid starvation for Eno1p, Fba1p and Tpi1p through their respective 5 UTR sequence. The strongest effects were observed for the unstructured and A-rich TPI1-5 UTR. Bioinformatical analyses helped to determine these features to be beneficial for an efficient trans

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