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Article10.1161/circ.120.suppl_18.s1125-d

Abstract 5603: LL-37 Improves Adhesion and Therapeutic Effects of Embryonic EPCs in Chronic Ischemia (Rabbit Model)

Achim Pfosser,Rabea Hinkel,Georg Stachel,Teresa Trenkwalder,Antonis K. Hatzopoulos,Robert Bals+1 more-2009-11-03-Circulation
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TL;DRAbstract

Therapeutic neovascularization can be achieved by vascular growth factors or by mobilisation or exogenous application of endothelial progenitor cells. We could already demonstrate that murine embryonal EPCs (eEPCs) are capable of inducing therapeutic angiogenesis in a chronic hindlimb model (rabbit). Furthermore we could show that homing of these cells can be enhanced by transient overexpression of the pro-inflammatory transcription factor nfkb subunit p65. In the present study, we aimed at peptide activation of NF kappa b in the eEPC population. For this purpose, we used the cathelicidin (LL-37), which binds to the receptor FPRL1 subsequently activating NF kappa B. Methods: Adhesion of wildtype (wt), p65 transfected (p65t) and LL37-activated eEPCs (100 μ g, LL37-eEPC) was examined in vitro in flow chamber experiments. In rabbits, at d7 after femoral artery excision, 5×106 eEPCs (n=5 per group) were retroinfused into the anterior tibial vein. At d7 and 35 angiography of both hindlimbs

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Therapeutic neovascularization can be achieved by vascular growth factors or by mobilisation or exogenous application of endothelial progenitor cells. We could already demonstrate that murine embryonal EPCs (eEPCs) are capable of inducing therapeutic angiogenesis in a chronic hindlimb model (rabbit). Furthermore we could show that homing of these cells can be enhanced by transient overexpression of the pro-inflammatory transcription factor nfkb subunit p65. In the present study, we aimed at peptide activation of NF kappa b in the eEPC population. For this purpose, we used the cathelicidin (LL-37), which binds to the receptor FPRL1 subsequently activating NF kappa B. Methods: Adhesion of wildtype (wt), p65 transfected (p65t) and LL37-activated eEPCs (100 μ g, LL37-eEPC) was examined in vitro in flow chamber experiments. In rabbits, at d7 after femoral artery excision, 5×106 eEPCs (n=5 per group) were retroinfused into the anterior tibial vein. At d7 and 35 angiography of both hindlimbs

Keywords

MedicineAngiogenesisNeovascularizationTherapeutic angiogenesisPopulationProgenitor cellIn vivoHindlimb

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