Successful Gene Transfer in Plants Using Electroporation and Electrofusion

TL;DRAbstract

Electroporation and electrofusion of plant tissues are rapid, reliable, techniques for genome modification. To accomplish these procedures two different wave forms have been developed and successfully used in plants during the last decade. We have shown that both square and exponential wave pulses are capable of incorporating genetic material into tobacco protoplasts. The production of an insect resistant potato hybrid, verified by phenotypic and genetic traits, has demonstrated the utility of electrofusion by the combination of two separate genomes. Specific genomic modifications are also possible as shown by the electroporation of marker genes into germinating tobacco pollen. The impact of these continuing investigations into the biological effects of electrical fields on plant cells, will enhance our ability to genetically manipulate biological systems.

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