Alterations in zinc status affects DNA integrity and transcription factor binding in vivo

TL;DRAbstract

There is increasing evidence that micronutrient deficiencies, such as zinc, may damage DNA. In the current study, we examined the effects of zinc status on DNA damage and transcription factors in both human and rat. In the human study, healthy adult males were first given a zinc adequate diet (11mg/d) for 13d to ensure normal baseline zinc. For the zinc depletion period, subjects were given a liquid diet (0.6mg/d) for 7d, and then given a low zinc diet (4mg/d) for an additional 35d. Finally, subjects were repleted with adequate zinc (11mg/d) diet for 28d, with supplemental zinc (20mg/d) given for the first 7d. At the end of each phase, blood cell DNA damage was assessed by comet assay. After zinc depletion, there was no significant change in DNA damage. However, after zinc repletion, there was a 40% decrease in DNA damage compared to baseline. To further examine mechanisms, we measured DNA damage and transcription factor function in zinc deficient rats. SD rats (n=12/group) were fed a

Chat with Paper

AI Agents for this Paper