Improvement of recombination activity mediated by conjugative relaxases

TL;DRAbstract

DNA processing during bacterial conjugation is driven by a nucleoprotein complex called relaxosome, where the relaxase is the key protein. Relaxases are site-and strand-specific endonucleases which recognize and cleave one strand of their cognate origin of transfer (oriT), and are transferred to the recipient cell covalently bound to the 5‘ end of the nicked DNA strand. TrwC is the relaxase of the conjugative plasmid R388. TrwC is also a site-specific recombinase capable of promoting efficient recombination between two cognate oriTs in the absence of conjugation (César et al., 2006). Moreover, it can integrate the conjugatively transferred DNA strand into its target sequence present in the recipient cell (Draper et al., 2005). TrwC is able to act as a recombinase and integrase usign as targets a discrete number of sequences, located in noncoding sites of the human genome (Agúndez et al., 2011; Agúndez et al., 2012). TraI is the relaxase of the F plasmid, highly related to TrwC, althoug

Chat with Paper

AI Agents for this Paper