The use of gibberellin mutants to explore the role of microtubules in stem elongation

TL;DRAbstract

Six single gene mutants of garden pea (Pisum sativum L.) were utilised to further examine the control of internode elongation. These short mutants could be divided into two types; GA-synthesis (1s, le), and GA-response ( 1k, lka, lkb, 1w) types. They were compared with the parental wild-type cv. Torsdag in an analysis of the changes in orientation of cortical microtubules in expanding dark-grown internode tissue with distance from the apical hook. Immunofluorescence analysis of FITC-labelled cortical microtubules in tangential sections of epidermal cells and longitudinal sections of subepidermal cortical cells of the mutants and wild-type plants revealed a shift away from the wild-type microtubule arrangement toward less transverse microtubule arrays in the mutants. This lower average microtubule orientation (resulting from a reduced proportion of cells with predominantly transverse microtubule arrangement) correlated with decreases in the rate and distribution of extension growth alon

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