Anion channels in roots : a role in sulphate transport.

TL;DRAbstract

The efflux of both organic and inorganic anions from plant roots plays an important role in plant nutrition. We are using the patch-clamp technique to investigate anion channel activity in the peripheral cells of Arabidopsis roots which are at the soil:rhizosphere interface. Plant were grown on luxurious sulphate supply (1.7 mM) for 7–10 days and the patch clamp technique was applied to root protoplasts derived from the epidermal cells of the elongation zone and young root hairs. To study sulphate- efflux currents, the pipette was loading with a medium containing 25 mM Cs2sulphate, 1 mM MgATP, 5 mM EGTA, 10 mM HEPES, pH 7.2, and the protoplast bath solution contained 0.5 mM LaCl3, 10 mM CaCl2, 5 mM MgCl2, 10 mM MES, pH 6.0. In the whole-cell configuration, all cells examined showed sulphate efflux currents which were time-dependent, inward rectifying and showed an average maximum peak current density of approximately 30"3 pAypF at approximately y90 mV. These currents could be partially

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