Transcription factor C/EBP‐beta is required for the regulation of apoptoic gene, the death‐associated protein kinase‐1 (DAPK1)

TL;DRAbstract

CAAAT/Enhancer binding protein (C/EBP) family of transcription factors plays a major role in regulating energy metabolism, immune response and cell differentiation. They are also suggested to have antitumor functions, although the mechanisms are unclear. Interferons are potent inhibitors of cell growth. A gene expression profiling in a cell line lacking the C/EBP‐β revealed that C/EBP‐β was required for the expression of a number IFN regulated genes, including, DAPK1. DAPK1 is a ca+2/calmodulin regulated serine/threonine kinase and a major tumor suppressor gene. Its expression is lost in multiple tumor types, although the mechanisms are unclear. Therefore, we investigated the regulatory influence of DAPK1 gene. We have isolated the gene promoter of DAPK1 and studied its regulation. ChIP assays showed that C/EBP‐β binds to the promoter of DAPK1. Mutation of a consensus C/EBP binding site (CBS) in the promoter did not decrease the inhibit gene expression. Therefore, we searched for poten

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