Flow cytometry, fluorescent probes, and flashing bacteria

TL;DRAbstract

Key words: fluorescent probes, flow cytometry, CSLM, viability, survival, microbial physiology, lactic acid bacteria, Lactococcus lactis , Lactobacillus plantarum , cheese, milk, probiotic In food industry there is a perceived need for rapid methods for detection and viability assessment of microbes. Fluorescent staining and flow cytometry provide excellent tools for microbial analysis. This thesis describes fluorescent techniques for assessment of the physiological state of lactic acid bacteria. Lysis of lactic acid bacteria plays a crucial role in cheese manufacturing. It is generally considered that lysis results in leakage of intracellular enzymes in the cheese curd and, thus, plays an important role in ripening and flavor formation. Bac Light (Molecular Probes) was applied for monitoring the lysis process of Lactococcus lactis MG1363 in a buffered suspension with high osmolarity to mimic cheese conditions. The Bac Light kit combines the nucleic acid dyes propidium iodide (PI) and

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