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The detection of potential drug resistance markers using proteomic technologies

Lisa Murphy-2006-01-01-Arrow@dit (Dublin Institute of Technology)
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TL;DRAbstract

This thesis sets out to increase our knowledge of mechanisms by which lung cancer cells develop resistance to chemotherapeutic agents. To further investigate drug resistance in lung cancer, a panel of four cell lines were chosen for pulse-selection with chemotherapeutic agents. The cell lines include two squamous (SKMES-1 and DLRP) and two small cell lung carcinoma (DMS-53 and NCI-H69) cell lines. The chemotherapy naive cell lines were pulse-selected rather than continuously selected with clinically relevant concentrations of taxotere, taxol, carboplatin and/or VP-16. The resulting twelve novel cell lines were tested for resistance to a cross-section of chemotherapeutic agents, for changes in invasiveness, motility, adhesiveness and for expression of the multidrug resistance (MDR) efflux pump proteins, P-gp and MRP-1. The SKMES-taxane selected variants were chosen for further analysis because the resistance profile was unstable and the concentration of drug used for selection was at a

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This thesis sets out to increase our knowledge of mechanisms by which lung cancer cells develop resistance to chemotherapeutic agents. To further investigate drug resistance in lung cancer, a panel of four cell lines were chosen for pulse-selection with chemotherapeutic agents. The cell lines include two squamous (SKMES-1 and DLRP) and two small cell lung carcinoma (DMS-53 and NCI-H69) cell lines. The chemotherapy naive cell lines were pulse-selected rather than continuously selected with clinically relevant concentrations of taxotere, taxol, carboplatin and/or VP-16. The resulting twelve novel cell lines were tested for resistance to a cross-section of chemotherapeutic agents, for changes in invasiveness, motility, adhesiveness and for expression of the multidrug resistance (MDR) efflux pump proteins, P-gp and MRP-1. The SKMES-taxane selected variants were chosen for further analysis because the resistance profile was unstable and the concentration of drug used for selection was at a

Keywords

TaxaneCarboplatinDrug resistanceCell cultureMitoxantroneMultiple drug resistanceBiologyCancer research

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