TL;DRAbstract
Current evidence indicates there exists two populations of neuronal nicotinic acetylcholine receptors, the $ alpha$-bungarotoxin ($ alpha$-BGT) sensitive and $ alpha$-BGT insensitive receptors. While the latter are thought to be involved in neurotransmission, the physiological significance of the former are not fully understood. However, evidence suggests that the toxin sensitive receptors may mediate trophic functions. The present studies were carried out to develop and validate novel and unique model systems for studying the structure, function and regulation of the nicotinic $ alpha$-BGT receptor. To this end, two such systems are presented here. First, the immortalized rat hippocampal H19-7 cell line was investigated. This cell line was shown to express a similar set of mRNA encoding for nicotinic receptor subunits as adult rat hippocampus. Further, binding studies using ($ sp{125}$I) -$ alpha$-BGT and other nicotinic ligands demonstrated a similar pharmacological profile between $
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Current evidence indicates there exists two populations of neuronal nicotinic acetylcholine receptors, the $ alpha$-bungarotoxin ($ alpha$-BGT) sensitive and $ alpha$-BGT insensitive receptors. While the latter are thought to be involved in neurotransmission, the physiological significance of the former are not fully understood. However, evidence suggests that the toxin sensitive receptors may mediate trophic functions. The present studies were carried out to develop and validate novel and unique model systems for studying the structure, function and regulation of the nicotinic $ alpha$-BGT receptor. To this end, two such systems are presented here. First, the immortalized rat hippocampal H19-7 cell line was investigated. This cell line was shown to express a similar set of mRNA encoding for nicotinic receptor subunits as adult rat hippocampus. Further, binding studies using ($ sp{125}$I) -$ alpha$-BGT and other nicotinic ligands demonstrated a similar pharmacological profile between $
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