Effect of isoacids on some rumen enzymes

TL;DRAbstract

Four total mixed rations prepared from fi nger millet straw as a roughage (48%) and mixed concentrate (52%), supplemented with 1 or 1.5% isoacids and protein (groundnut cake, 5% crude protein more than control) were given in a change-over experiment to sheep. Biochemical activities were estimated for enzymes e.g., urease, cellulase, protease, and amylase, in various fractions of rumen fl uid. Rumen samples were fractionated by centrifugation in strained rumen fl uid without protozoa (SRFWP), cell free rumen fl uid (CFRF) and enzymes associated with the bacterial cell (EABC). Samples of SRFWP and EABC contained higher enzyme activity than CFRF. These values showed very close cooperative action between proteolytic and amylolytic bacteria under the experimental condition, or perhaps presence of some species of bacteria with both activities. Results showed isoacid and crude protein enhanced microbial function (P<0.05) and this can change the pattern of enzymes in the rumen of sheep.

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