乙型脑炎病毒结构蛋白C+pre M和E基因的克隆与序列分析

TL;DRAbstract

Objective To conduct amplification, cloning and sequencing of Japanese encephalitis virus (JEV structural protein C+pre M and E genes. Methods Two pairs of primers were used for amplifying Japanese encephalitis virus (JEV) structural protein C+pre M and E genes with RT-PCR in suckling mice brain infected with 1EV Nakayama-NIH strain. The amplified fragments were cloned into the pGEM-T easy vector and transformed E. coli DH5. The recombinant plasmid were identified by electrophoresis, PCR, and endonuclease analysis, and confirmed by sequencing. The nucleotide sequences were compared with the structural protein C+pre M and E genes of other nine reference strains. Results The nucleotide homology of JEV Nakayama-NIH strain with other published JEV structural protein C+pre M and E genes was between 96%-99%. Conclusion Characterization of the JEV Nakayama-NIH strain at the genetic identification can be an important step toward identifying JEV in miniature pigs, and may aid in the design of J

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