Real-time PCR技术结合浓缩涂片染色法用于结核病快速诊断的研究

TL;DRAbstract

Objective: To evaluat the applicative value of applying Real time PCR technology and concentration smear staining method to rapid diagnosing tuberculosis. Methods: To apply Real-time PCR technology and concentration smear staining method to check 1052 pieces of clinic specimen, and compare the result with those of direct smear method and BACTEC MGIT-960 rapid cultivation method. Results: The positive relevance ratio of Real-time PCR technology combining with concentration smear staining method was 30.6%, which was evidently higher than that of direct smear method of 19.2% (x^2=36.6, P＜0.01) and closes to BACTEC-960 cultivation method of 34.9% (x^2=2.5, P＞0.05). Moreover, the testing time was evidently shortened as 4 hours. At the standard of evaluation method of BACTEC MGIT-960 cultivation result, the sensitivity of Real-time PCR technology combining with concentration smear staining method was 89.7% (280/312), the specificity was 94.3% (698/740) and the agreement was 93.0% (978/1052).

Chat with Paper

AI Agents for this Paper