Methods for analysis of dietary fibre - advantageand limitations

TL;DRAbstract

The two main approaches for the determination of dietary fibre (DF) in food and feedstuffs are the enzymatic-and nonenzymatic-gravimetric AOAC (Association of Official Analytical Chemists) procedures and the enzymatic-chemical Englyst and Uppsala procedures. The main analytical problems which have been shown to influence the performance of the enzymatic-gravimetric AOAC procedures are: variable starch removal, too high final phosphate buffer concentration, and hence variable ash content of gravimetric residues, problems with residual nitrogen determination, filtration problems of viscous and desugared fruit samples, and variable blank values. Standardisation and improvements of the methods have gradually improved the performance of the gravimetric procedures to acceptable levels. The enzymatic-chemical methods with gas-liquid chromatography (GLC), high performance liquid chromatography (HPLC) or colorimetry as endpoint determination are generally more complex, involve more steps and re

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