Methods to detect bacterial contamination of blood products

TL;DRAbstract

Bacterial contamination of blood products presents an ongoing challenge to transfusion therapy. Contaminating bacteria are typically introduced in low numbers at the time of collection, but can proliferate during storage to reach >10‚ÄövÖœÄ colony forming units/mL (CFUhnL). The consequences of contamination include product wastage and transfusion-transmitted sepsis, but in Australia screening is conducted on only 1% of products by a slow and labour-intensive culture method. Accordingly, there is a need for a more practicable and rapid assay. The purposes of this study were to investigate the growth kinetics of bacteria in stored products, and to develop a rapid method to detect them. Pseudomonas, Staphylococcus and Yersinia species were inoculated into buffy coat platelet concentrates (PCs) and red cell concentrates (RCCs) at 10, 10¬¨‚⧠or 10¬¨‚â• CFU/mL and then stored at 22 ¬¨¬± 2¬¨‚àûC, agitated for PCs, or at 4 ¬¨¬± 2¬¨‚àûC, stationary for RCCs. Bacterial growth was monitored b

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