Metal-oxide enrichment and gas-phase characterization of sulfopeptides using Fourier transform ion cyclotron resonance mass spectrometry

TL;DRAbstract

Though not as well studied as phosphopeptides, sulfopeptides are important for many biological processes, including proper endocrine function and extracellular signaling. The discovery of sulfopeptides dates back to the 1920s; however, their enrichment and characterization have only recently become of broader interest. With a limited toolbox for analyzing sulfopeptides, we employ several chemistries to develop robust enrichment and characterization methods. At the heart of each method lies Fourier transform ion cyclotron resonance mass spectrometry, a gas-phase detection method with the power to differentiate even the slightest mass differences, such as phosphate vs. sulfonate. First, Lewis acid-base characteristics inherent to transition metal oxides are examined for the selective interaction and enrichment of sulfopeptides in the presence of mixtures of competing poly-oxyanions. Careful control of the binding and elution pH with an optimized amount of sulfopeptide loaded onto the met

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