Primary Trisomics in Soybean: Origin, Identification, Breeding Behavior, and Use in Linkage Mapping

TL;DRAbstract

A primary trisomic is an excellent cytogenetic tool for locating Mendelian genes on a particular chromosome and for associating a conventional genetic linkage group with a specific chromosome. The objectives of this study were to produce and identify 20 primary trisomics of soybean [ Glycine max (L.) Merr.] and use them for associating linkage groups with specific chromosomes. Primary trisomics isolated from the progenies of asynaptic and desynaptic mutants, male sterile lines, neutron‐irradiated plants, and tissue culture‐induced sterile mutants were backcrossed (BC 4 ) with soybean cv. Clark 63. They were identified at pachytene on the basis of the diagnostic landmarks such as association of the extra chromosome with its normal homologues in a trivalent configuration, distribution of euchromatin and heterochromatin, total chromosome length, arm ratios, and by examining chromosome pairing at metaphase I in F 1 plants with 2 n = 42 chromosomes isolated from crosses among primary trisom

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