Untersuchung sub-mitochondrialer Proteinverteilungen in unbehandelten und apoptotischen humanen Zellen mittels STED-Mikroskopie

TL;DRAbstract

Mitochondria are essential for eukaryotic cells. These organelles exhibit a complex internal structure and fulfil a variety of different tasks. They are known as the powerhouses of the cell because of their important role in the supply of the universal biological energy equivalent ATP. Although mitochondria belong to the best characterized organelles, only little is known about protein distributions within mitochondria. The light microscopic analysis of sub-mitochondrial protein distributions was neglected for a long time, because mitochondria have a diameter of 200-400 nm, which is in the range of the resolution limit of conventional light microscopes. However, recent developments of high resolution light microscopic techniques like STED-microscopy allow the investigation of sub-mitochondrial protein distributions. Up to now, only qualitative analysis of mitochondrial proteins and their distributions were performed. In this work STED-microscopy was combined with mathematical algorithm

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