In vitro Propagation of Kainth (Pyrus pashia) Using Explants from Forced Cutting

TL;DRAbstract

This study was carried out in Tissue culture laboratory, Department of Fruit Science, Punjab Agricultural University, Ludhiana during 2011-13. The effect of various media {1/2 MS (M1), MS (M2) and WPM (M3)} and growth regulators (BAP, IBA and NAA) on establishment, proliferation and rooting was studied. Maximum establishment (63.60%) was obtained on M2 containing BAP (1.5 mgl-1) and IBA (0.25 mgl-1). Maximum proliferated cultures (95.30%) and shoots per explant were obtained using M3 medium fortified with BAP (3.0 mgl-1). However, shoots of maximum length (42.97 mm) were obtained in M3 medium containing BAP (0.0 mgl-1) i.e control. Rooting (%), roots per explant and root length was found to be influenced by type of medium and growth regulator fortification. Rooting (%) was maximum (13.34%) was observed in M1 medium supplemented with IBA (0.1 mgl-1). However, NAA (1.0 mgl-1) induced rooting of 29.61 per cent using M1 medium. Maximum roots per explant were obtained using M1 medium supple

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