TL;DRAbstract
Staphylococcal enterotoxin B (SEB), an exotoxin produced by staphylococcal aureus , and lipopolysaccharide (LPS), an endotoxin released from bacterial cell walls, produce similar flu‐like symptoms. By comparing human genetic responses to these toxins, a set of genes can be identified to facilitate rapid diagnosis and potentially prevent lethal shock due to prolonged toxin exposure. SEB‐infected and LPS‐infected RNA samples were gathered from PBMC cells at several time points, between 2 and 72 hours. Gene expression patterns specific for both toxins were determined by microarray analysis and critical genes were identified by GeneSpring analysis software. A set of these genes was selected for analysis based on function, such as apoptosis, cytokine induction, phosphorylation or transporter activity. After designing primers for the target genes, real time PCR and RT‐PCR were used to confirm the gene expression pattern initially established by microarray analysis. The combination of microar
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Staphylococcal enterotoxin B (SEB), an exotoxin produced by staphylococcal aureus , and lipopolysaccharide (LPS), an endotoxin released from bacterial cell walls, produce similar flu‐like symptoms. By comparing human genetic responses to these toxins, a set of genes can be identified to facilitate rapid diagnosis and potentially prevent lethal shock due to prolonged toxin exposure. SEB‐infected and LPS‐infected RNA samples were gathered from PBMC cells at several time points, between 2 and 72 hours. Gene expression patterns specific for both toxins were determined by microarray analysis and critical genes were identified by GeneSpring analysis software. A set of these genes was selected for analysis based on function, such as apoptosis, cytokine induction, phosphorylation or transporter activity. After designing primers for the target genes, real time PCR and RT‐PCR were used to confirm the gene expression pattern initially established by microarray analysis. The combination of microar
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