Interleukin 2 mRNA Induction in Human Lymphocytes: Analysis of the Synergistic Effect of a Phorbol Ester and Phytohemagglutinin1

TL;DRAbstract

Induction of interleukin 2 (IL2) mRNA synthesis in human tonsillar lymphocytes was studied by quantifying the relative levels of IL2 mRNA in the lymphocytes stimulated under various conditions by the dot hybridization method. A remarkable increase of IL2 mRNA was induced by stimulation with phytohemagglutinin (PHA) in the presence of 12-O-tetradecanoyl-phorbol-13-acetate (TPA). The kinetic study revealed that the IL2 mRNA level of the lymphocytes increased from 2 h of culture, reached a maximal level at 12 h, maintained a relatively high level until 48 h and then sharply decreased by 72 h after the stimulation. Inhibition experiments with actinomycin D showed that the increase was due to a transient synthesis of the mRNA after the stimulation, which almost stopped by 12-16 h. DNA synthesis and cell division were not necessary for the induction of IL2 mRNA production but the induction was inhibited by dexamethasone, showing that the production was mainly associated with the G1 phase of

Chat with Paper

AI Agents for this Paper