Comparative analyses of antigenic and enzymatic characteristics of the genus Trametes (Polyporaceae).

TL;DRAbstract

The taxonomy of the Polyporaceae with emphasis on Trametes versicolor and related species has been studied using comparative analyses of antigenic and enzymatic characteristics of 60 polypore cultures. The system of laccase (p-diphenol: 02 oxidoreductase, E .0 .1.10.3 .2) isoenzymes produced by T. versicolor has been selected for detailed investigation. T. versicolor and some related species produced three laccase isoenzymes which have been named laccase 1, laccase 2 and laccase 3 according to their relative electrophoretic mobilities. The three isoenzymes exhibited identical affinity to catechin (Km = 10 -4M) and a similar substrate specifity, but they were differentially inactivated by sodium diethyldithiocarbamate, extreme H ion concentrations, and urea. At least five different antigenic determinants (a, b, c, d and e) may be present among the three laccases. Laccase 1 (determinants a and d) is antigenically distinct from laccase 2 (b and e). Both these isoenzymes are related to lac

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