Mean fluorescence intensities (MFI) of monocyte or cDC cytokine production drastically fell over the first year of life for all cytokines except TNF-α and IL-6 in response to LPS stimulation.

TL;DRAbstract

<p>Whole blood samples from ∼30 neonate-infant subjects were followed longitudinally from 2 weeks (2 wk; n = 28), 6 weeks (6 wk; n = 26), 6 months (6 mo; n = 23) and 12 months (12 mo; n = 20) of life, and 10 adults (Ad) were stimulated with the indicated TLR ligands for 6 h, and intracellular cytokine levels were measured by flow cytometry for TNF-α, IL-6 and IL-12/23p40 gated on monocytes (blue line) and cDC (red line). The MFI values from all samples of each age group were averaged after first excluding the samples that had cytokine-positive percentage <4% of the APC subtype. Means for each population were derived from the FlowJo software; error bars indicate SEM.</p>

Chat with Paper

AI Agents for this Paper