Characterization of Neisseria wadsworthii 9715 : phenotypic analyses and assess of the natural competence for transformation of the Neisseria species Neisseria wadsworthii

TL;DRAbstract

The bacterium Neisseria wadsworthii (Nw) belong to the genus Neisseria in the family Neisseriaceae.Two novel strains of Nw, isolated from clinical specimens have been identified and have been only poorly characterized Neisseria species so far.N. meningitidis, the meningococcus (Mc) on the analysis were performed to observe the expression of PilE in Nw 9715.The analysis revealed that Nw expresses PilE which was detected with cross reactive antibodies directed against Mc pilin.The Matrix Assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometer (MS) based Biotyper database was extended with currently poorly or non-represented Neisseriaceae.Mass spectra from several Neisseria, available in the laboratory, together with Nw 9715 were obtained and edited with the Micro Flex Control setup program and were further calibrated and corrugated by applying the Flex Analysis program.The spectra for the strains were successfully added to the Biotyper 3.1 database and the datab

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