Cytogenetic and molecular investigations of genomic instability related to human cellular aging

TL;DRAbstract

Both an investigation on in vitro aging of human fibroblasts (IMR-90 and IMR-91) and a longitudinal study of various individuals, each at two different ages, were systematically carried out for age related chromosomal anomalies. Chromosome-specific aneuploidy analyses of various cell samples were performed by FISH with 17 centromeric DNA probes. Our data on total aneuploidy in young IMR-90 and IMR-91 cells indicated that significantly higher proportions of cells with aneuploidy could be detected at interphase than at metaphase. In contrast, senescent cells at interphase showed significantly lower aneuploidy values as compared to that of young cells at interphase. Our study shows that, cellular dynamics with respect to aneuploidy differs significantly at interphase and at mitosis during in vitro aging of human fibroblasts, and we propose a "Selection Hypothesis" as an explanation to our findings. We have also performed a longitudinal study on both numerical and structural chromosome ana

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