Abstract 18960: Ciliary Neurotrophic Factor Increases the Angiogenic Properties of Human Pluripotent Stem Cell Derived Neuropilin-1+ CD34+ Nascent Endothelial Cells Via a JAK2 Dependent Pathway

TL;DRAbstract

Pluripotent stem cells are a model to identify novel regulators of angiogenesis. Previously, we showed that Neuropilin-1 (NRP1) CD34- cells were vascular precursors. Subsequently, genomic analysis revealed that NRP1+ CD34+ cells had transcript content consistent with nascent endothelium, and unique growth factor receptors that may be exploited to modulate their angiogenic properties. The objectives of our study was to verify that NRP1+ CD34+ cells form endothelium, and determine the function of the Ciliary Neurotrophic Factor (CNTF) Receptor and its downstream kinase Jak2 in angiogenesis. Methods and Results: Human induced pluripotent stem cells (hiPSCs) were differentiated as embyroid bodies and NRP1+ CD34+ nascent endothelial cells were sorted. NRP1+ CD34+ cells readily formed sheets of endothelium expressing VE-Cadherin, CD146, PECAM, and eNOS, absorbed acetylated LDL, and formed tubule networks in Matrigel. These features confirmed their endothelial properties. Here we show that CN

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