Deep dermal up-regulation of HIF-1A is directly correlated with apoptosis in Spitz tumors

TL;DRAbstract

Background: The microvessel profile bases in cutaneous atypical melanocytic lesions (atypical melanocytic nevi, AMN, and Spitz tumors, ST) are poorly understood. No study has correlated microvessel profile and HIF-1 expression with cell kinetics by topographic compartments in AMN and ST to date. Design: We selected low-grade AMN (93), high-grade AMN (31), ST (42), malignant melanomas (42, 15 radial growth phase MM-RGP and 27 vertical growth phase MM- VGP), and conventional melanocytic nevi (15 junctional, 20 compound), the latter two groups used as controls. Immunostaining for Ki-67 and HIF-1, and in situ end labeling (ISEL) of DNA fragments (using Klenow fragment of DNA polymerase I) were scored by topographic compartments: junctional, superficial dermal (above 0.76 mm) and deep dermal (below 0.76 mm), screening the whole compartment in each case. Appropriate controls were run in each sample. CD-31-stained slides were used to estimate microvessel density. The results were statisticall

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