Highly pure, viable and functional intact human Natural Killer (NK) cells isolated in a single isolation step using Dynabeads® FlowCompTM Technology

TL;DRAbstract

BACKGROUND NK cells contribute to a variety of innate immune responses to viruses, tumors and allogeneic cells. In humans, NK cell express CD56. However, this surface marker is not exclusive for NK cells and separation technology focusing on this marker will potentially result in contamination of non‐NK cells or involve several isolation steps. Here we present a single step, positive isolation protocol for human NK cells. METHODS: Peripheral blood mononuclear cells (PBMC) were isolated from healthy individuals. NK‐specific antibody conjugated to a modified biotin was added and incubated at 2–8 °C. In the next step modified streptavidin coated Dynabeads were added and placed in the magnet after incubation at room temperature. After resuspension with FlowComp release buffer the bead‐free cells were analyzed on flow cytometry and tested in cytotoxicity assays. RESULTS: Highly pure human CD56 + CD3 − NK cells were isolated from healthy blood donors using Dynabeads® FlowComp™ Human NK Cell

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