TL;DRAbstract
The interaction of microbes with dendritic cells (DCs) is likely to have an enormous impact on the initiation of the immune response against a pathogen. In this study, we compared the interaction of Mycobacterium tuberculosis with murine bone marrow derived DCs and macrophages in vitro. M. tuberculosis grew equally well within non-activated DCs and MØ. Activation of DCs and MØ with IFN-g and LPS inhibited the growth of the intracellular bacteria in an NOS2-dependent fashion. However, while this activation enabled MØ to kill the intracellular bacteria, the M. tuberculosis bacilli within activated DCs were not killed. Thus, DC could restrict the growth of the intracellular mycobacteria, but were less efficient than macrophages at eliminating the infection. These results may have implications for priming immune responses to M. tuberculosis. In addition, they suggest that DCs may serve as a reservoir for M. tuberculosis in tissues, including lymph nodes and lungs. Dendritic cells (DC) poss
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The interaction of microbes with dendritic cells (DCs) is likely to have an enormous impact on the initiation of the immune response against a pathogen. In this study, we compared the interaction of Mycobacterium tuberculosis with murine bone marrow derived DCs and macrophages in vitro. M. tuberculosis grew equally well within non-activated DCs and MØ. Activation of DCs and MØ with IFN-g and LPS inhibited the growth of the intracellular bacteria in an NOS2-dependent fashion. However, while this activation enabled MØ to kill the intracellular bacteria, the M. tuberculosis bacilli within activated DCs were not killed. Thus, DC could restrict the growth of the intracellular mycobacteria, but were less efficient than macrophages at eliminating the infection. These results may have implications for priming immune responses to M. tuberculosis. In addition, they suggest that DCs may serve as a reservoir for M. tuberculosis in tissues, including lymph nodes and lungs. Dendritic cells (DC) poss
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