High Performance Liquid Chromatographic Determination of Malondialdehyde in Vegetable Oils

doi:https://doi.org/10.1093/jaoac/66.2.304

Open AccessArticle10.1093/jaoac/66.2.304

High Performance Liquid Chromatographic Determination of Malondialdehyde in Vegetable Oils

Teruhisa Hirayama,Naohide Yamada,Motoshi Nohara,Shozo Fukui-1983-03-01-Journal of AOAC INTERNATIONAL

TL;DRAbstract

A simple high performance liquid chromatographic (HPLC) method has been developed for determining malondialdehyde (MDA) in vegetable oils. MDA was reacted with dansyl hydrazine in an acidic medium, and the product, 1-dansyl-pyrazole, was determined by HPLC, using a Zorbax sil column with mixed mobile phase of n-hexane-methylene chloride. MDA can be determined as 1-dansyl-pyrazole by fluorometric detection at a level of 0.01 ppm in vegetable oils.

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A simple high performance liquid chromatographic (HPLC) method has been developed for determining malondialdehyde (MDA) in vegetable oils. MDA was reacted with dansyl hydrazine in an acidic medium, and the product, 1-dansyl-pyrazole, was determined by HPLC, using a Zorbax sil column with mixed mobile phase of n-hexane-methylene chloride. MDA can be determined as 1-dansyl-pyrazole by fluorometric detection at a level of 0.01 ppm in vegetable oils.

Keywords

ChromatographyChemistryHigh-performance liquid chromatographyMalondialdehydePyrazoleDansyl chlorideHydrazine (antidepressant)Hexane

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