Cytotoxic effects of capsaicin and capsicum extracts on neuroblastoma cells

TL;DRAbstract

Capsaicin, a hot pepper alkaloid, has been shown to stimulate the release of serotonin and dopamine in SH-SY5Y neuroblastoma cells. Binding on vanilloid receptor 1 (TRPV1) is one of the cellular mechanisms responsible for this effect. On the other hand TRPV1 are involved in cell proliferation and apoptosis. The aim of the present experiments was to investigate the effect of the potential anticancer agent capsaicin on B104 neuroblastoma cells. Materials and methods: MTT and LDH assays were used to determine viability and cell death in B104 neuroblastoma cells. Capsicum ethanolic extracts isolated from 4 different genotypes of hot species of Capsicum annuum L. and capsaicin solutions in different concentrations and time of exposures were investigated. Results: Capsaicin (500nM, 1 μM, 10μM) did not influence significantly viability or cell death of B104 cells when it was applied for 1 or 24 hours incubation. There was a significant cytotoxicity of high concentrations of capsaicin (100μM),

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