IN VIVO SISTER CHROMATID EXCHANGES: A TEST SYSTEM MONITORING ENVIRONMENTAL GENOTOXICITY.

TL;DRAbstract

An in vivo sister chromatid exchange (SCE) assay system for monitoring environmental genotoxicity, using bone marrow cells has been developed, tested and applied to groups of inbred and wild mice under various environmental conditions. The best results were obtained with nine, serial, intraperitoneal injections of 5-bromo-2'-deoxyuridine (BUdR) (40 (mu)g/g of body weight) and 5-fluorodeoxyuridine (FUdR) (2 (mu)g/g of b.w.) and then with 5 (mu)g/g of b.w. of colchicine, given on the 27th hour following the first injections of BUdR/FUdR. The mice were sacrificed 3 hours later. With the above injection schedule, male mice of C3H/HeJ and C57BL/6J maintained in the laboratory gave a mean (+OR-) SEM baseline SCE value of 3.42 (+OR-) 0.07 and 3.62 (+OR-) 0.08 respectively. The female mice of the same strains gave higher numbers of SCEs/cell. Males obtained from natural populations in southwestern Ontario had a higher mean SCE value (6.02 (+OR-) 0.16) as did the inbred males maintained in outd

Chat with Paper

AI Agents for this Paper