Regulation der Zuckerverwertung in Corynebacterium glutamicum

TL;DRAbstract

In this work the influence of the PTS-sugars glucose, fructose and sucrose on growth as well as on lysine- and glutamate production in C. glutamicum was investigated. Growth rates, biomass yields and ethambutol induced glutamate production showed no differences on glucose, fructose or sucrose. In contrast, the lysine yield of C. glutamicum DM1730 on glucose was more than twofold increased as compared to the other tested sugars. Overexpression of the malic enzyme gene to improve the NADPH supply had no effect on the lysine yield whereas overexpresion of the fructose-1,6-bisphosphatase (fbp) gene resulted in a doubling of the lysine yield on sucrose. This result could be explained by the observation of a decreased intracellular concentration of fructose-1,6-bisphosphate, a competive inhibitor of the key enzymes of the pentose phopshate pathway, apon overexpression of fbp. That probably led to a higher carbon flux through the pentose phosphate pathway and thus to a better NADPH supply. A

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