Label-free biosensor arrays using open circuit potential measurements

TL;DRAbstract

Electrochemical sensors can be miniaturised more readily than optical sensors and are suitable for use with portable instrumentation by minimally trained clinical personnel. A real-time measuring system could provide detailed understanding of complex biological systems. Label-free high throughput analysis methods allowing protein/DNA quantitative detection is highly desirable. In particular field-effect devices are promising candidates for the development of inexpensive microarrays associated with portable instrumentation. These stable semiconductor devices measure variations in the open circuit potential (OCP) that occur at the metal gate interface when the charge density and distribution of the immobilised biolayer changes upon interaction with a bioconjugate. We here present a multi-channel biosensor using direct multiplexed OCP variation measurements. The OCP was measured in real time using an ultra-low input bias current instrumentation amplifier providing an accurate differential

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