Live imaging of autoimmune responses in distinct milieus of the central nervous system

TL;DRAbstract

Multiple sclerosis (MS) is the most common inflammatory disease of the central nervous system (CNS). Even though it is considered to be a classical demyelinating disease, an increasing body of evidence also demonstrates the early presence of inflammatory lesions in the grey matter. In experimental autoimmune encephalomyelitis (EAE), the animal paradigm of MS, preferential involvement of grey matter is rarely reported, hindering the study of this important aspect of the disease. In this thesis, green fluorescent protein (GFP)-transduced effector T cells reactive either against a myelin antigen, i.e. the myelin basic protein (TMBP-GFP) or against a neuronal antigen, i.e. the β-synuclein (Tβ-Syn-GFP) were used to induce EAE by passive transfer in recipient Lewis rats. The aim was to elucidate the homing behaviour of encephalitogenic effector T cells into the distinct milieus of the CNS. Both of these T cell lines had a similar phenotype in vitro and similar pathogenic potential in vivo. H

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