<i>Spink9</i> mRNA expression in human skin and keratinocytes.
TL;DRAbstract
<p>(A) Expression profile of <i>Spink9</i> mRNA. Fragments were obtained after RT-PCR amplification on human multiple tissue cDNAs with primers specific to the human <i>GAPDH</i> and <i>Spink9</i>. Lanes are labelled according to the template tissue. The <i>Spink9</i> fragments are of 175 bp in size. H<sub>2</sub>O (no cDNA) and RT-control (no RNA template) were used as negative controls. (B) <i>Spink9</i> mRNA expression in cultured primary keratinocytes. Quantitative realtime PCR was conducted on RT-PCR products of total RNA samples collected from keratinocytes treated with 1.0 mM CaCl<sub>2</sub> for the indicated time. Bar graphs represent the relative mRNA expression of <i>Spink9</i> against <i>GAPDH</i>. Data are obtained from three independent experiments with different sources of keratinocytes and are indicated as the mean+/−SD. * indicates significant (p<0.05);
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<p>(A) Expression profile of <i>Spink9</i> mRNA. Fragments were obtained after RT-PCR amplification on human multiple tissue cDNAs with primers specific to the human <i>GAPDH</i> and <i>Spink9</i>. Lanes are labelled according to the template tissue. The <i>Spink9</i> fragments are of 175 bp in size. H<sub>2</sub>O (no cDNA) and RT-control (no RNA template) were used as negative controls. (B) <i>Spink9</i> mRNA expression in cultured primary keratinocytes. Quantitative realtime PCR was conducted on RT-PCR products of total RNA samples collected from keratinocytes treated with 1.0 mM CaCl<sub>2</sub> for the indicated time. Bar graphs represent the relative mRNA expression of <i>Spink9</i> against <i>GAPDH</i>. Data are obtained from three independent experiments with different sources of keratinocytes and are indicated as the mean+/−SD. * indicates significant (p<0.05);
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