Mutagenic Potential of Municipal Sewage Sludge and Sludge Amended Soil

doi:https://doi.org/10.1520/stp26718s

Mutagenic Potential of Municipal Sewage Sludge and Sludge Amended Soil

K.C. Donnelly,KW Brown,C.P. Chisum-1988-01-01

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TL;DRAbstract

Twelve municipal wastewater treatment plant sludges were collected and extracted by sequential extraction with methylene chloride and methanol on a Soxhlet apparatus. Each of three sludge fractions, methylene chloride, methanol, and a combined fraction (36 fractions total) was tested in the Salmonella/microsome assay. Only one of the sludge fractions induced a positive response in the absence of metabolic activation, and twelve fractions induced a doubling of revertant colonies at two or more consecutive dose levels with activation. The maximum mutagenic response observed with strain TA98 in a methylene chloride fraction was 120 net revertants at a dose level of 10 mg of extract per plate. The maximum response induced by the methanol and combined fractions was 89 net revertants, induced by a municipal sludge with 20% industrial contribution, and 61 net revertants, induced by a municipal sludge with 19% industrial contribution, respectively, both tested with strain TA98 at a dose level

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Twelve municipal wastewater treatment plant sludges were collected and extracted by sequential extraction with methylene chloride and methanol on a Soxhlet apparatus. Each of three sludge fractions, methylene chloride, methanol, and a combined fraction (36 fractions total) was tested in the Salmonella/microsome assay. Only one of the sludge fractions induced a positive response in the absence of metabolic activation, and twelve fractions induced a doubling of revertant colonies at two or more consecutive dose levels with activation. The maximum mutagenic response observed with strain TA98 in a methylene chloride fraction was 120 net revertants at a dose level of 10 mg of extract per plate. The maximum response induced by the methanol and combined fractions was 89 net revertants, induced by a municipal sludge with 20% industrial contribution, and 61 net revertants, induced by a municipal sludge with 19% industrial contribution, respectively, both tested with strain TA98 at a dose level

Keywords

Sewage sludgeSewage sludge treatmentEnvironmental scienceSewage farmWaste managementSewageEnvironmental engineeringSewage treatment

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