Cryopreservation of mesenchymal stem cell and tissue engineered constructs using non-toxic cryoprotective agents

TL;DRAbstract

The thesis work deals with the development of cryopreservation strategy for long term storage of MSCs and MSCs seeded tissue engineered constructs using non toxic cryoprotective agents as freezing medium. In the first phase, different freezing medium consisting of a combination of natural extracellular cryoprotectants namely trehalose, hydroxyl ethyl starch, polyvinyl pyrolidine and intracellular CPAs like erythritol, taurine and ectoin were used for cryopreservation of MNCs following the Taguchi Orthogonal Array method. Among the various combinations, freezing medium consisting of trehalose (0.05mM), ectoin (0.10mM) and catalase (100μg/ml) has shown maximum MNCs viability. These CPAs were further investigated individually as well as in combination to see their effectiveness towards long term preservation of MSCs. Among the freezing solutions, solution prepared using trehalose (0.3mM), ectoin (0.3mM), and catalase (100μg/ml) was found to be the most effective in preserving MSCs in long

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