Characterisation of ATP-binding cassette (ABC) transporters in the Calu-3 human bronchial epithelial cell culture model

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Characterisation of ATP-binding cassette (ABC) transporters in the Calu-3 human bronchial epithelial cell culture model

Victoria Hutter,Constanze Hilgendorf,Alan Brown,Anne Cooper,Vanessa Zann,David Pritchard+1 more-2010-10-01-University of Hertfordshire Research Archive (University of Hertfordshire)

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TL;DRAbstract

Expression and functionality of ATP-binding cassette (ABC) transporters were assessed in Calu-3 cell monolayers cultured at an air-liquid interface. Transporter gene expression was in agreement with published data in human lungs with the exception of BSEP which was over-expressed in Calu-3 cells. Net secretory transport of the ABC substrate 3H-digoxin was reduced in presence of the inhibitors verapamil, PSC833 and MK571. However, the transporter(s) involved could not be identified.

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Expression and functionality of ATP-binding cassette (ABC) transporters were assessed in Calu-3 cell monolayers cultured at an air-liquid interface. Transporter gene expression was in agreement with published data in human lungs with the exception of BSEP which was over-expressed in Calu-3 cells. Net secretory transport of the ABC substrate 3H-digoxin was reduced in presence of the inhibitors verapamil, PSC833 and MK571. However, the transporter(s) involved could not be identified.

Keywords

ATP-binding cassette transporterCell cultureHuman cellChemistryTransporterCell biologyBiologyBiochemistry

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