Histochemical localization of kallikrein granules in the submaxillary gland of the guinea-pig.

TL;DRAbstract

Subcellular kallikrein-containing granules have been isolated from homogenates of mammalian submaxillary glands by differential and density-gradient centrifugation (Bhoola & Ogle, 1966; Bhoola, 1968, 1969). The present study is an attempt to localize these granules in the intact gland by histochemical techniques. Histologically, the guinea-pig submaxillary gland is believed to consist of serous acini only (Schackleford & Klapper, 1962) and thus is particularly suitable for such a study since, when examined by light microscopy, the acinar cells appear morphologically homogeneous (cf. Spicer & Duvenci, 1964). In view of the fact that kallikrein is a glycoprotein (Trautschold, Fritz & Werle, 1966) and kallikrein-containing granules isolated by density-gradient centrifugation react positively with the periodic acid-Schiff reagent (PAS) (Bhoola, 1968) the main histochemical characterization in this study was based on affinity of material in the granules for PAS. A preliminary account of the

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