Genetic targeting and analysis of Parvalbumin and VGLUT3 expressing inhibitory neurons

TL;DRAbstract

GABAergic interneurons are a highly diverse class of cell types that play essential roles in regulating the input/output behaviour of pyramidal neurons and of other interneurons. Thereby, these cells play a role in shaping neuronal network patterns and oscillations that are involved in higher brain functions like memory, attention and perception. Furthermore, GABAergic signalling is thought to play a crucial role in the development of single neurons and of neuronal networks.A genetic approach was used to analyse the contribution of subsets of GABAergic interneurons to inhibitory neurotransmission. Mice carrying a loxP-flanked allele of the vesicular inhibitory amino acid transporter (Viaat) were crossed to mice expressing Cre recombinase under the control of the Parvalbumin (PV) and vesicular glutamate transporter 3 (VGLUT3) promoters. Since Viaat is essential for neurotransmitter release in GABAergic neurons, this approach allows the selective silencing of GABAergic inhibitory neurons

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