Protein intake is essential to increase S6K1 phosphorylation following exercise

TL;DRAbstract

Objective: To determine the impact of protein co‐ingestion before and after exercise on S6K1 and S6 phosphorylation status in human muscle. Design: Before, immediately after and 1h after resistance exercise, subjects consumed 0.3 g·kg −1 carbohydrate with or without 0.15 g·kg −1 protein (CHO and CHO+PRO test, respectively). Muscle biopsies were taken before and immediately after exercise and after 1 and 4h of recovery to determine 4E‐BP1, S6K1 (T 389 ) and S6 phosphorylation status in skeletal muscle tissue. Results: Following resistance exercise, 4E‐BP1 phosphorylation status was reduced to a greater extent in the CHO vs. CHO+PRO test (−48±7 vs. −15±14%, respectively; P<0.01). During recovery, 4E‐BP1 phosphorylation status increased in both trials (P<0.01) and tended to be higher in the CHO+PRO test (P=0.08). In contrast to the CHO test, S6K1 phosphorylation at T 389 was increased following exercise in the CHO+PRO test (−4±2 vs. +78±2%, respectively; P<0.01). During recovery,

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